Using nystatin perforated-patch whole cell recording, we investigated the role of Cl⁻conductance in the modulation of outer medullary descending vasa recta (OMDVR) pericyte membrane potential (Ψm) by ANG II. ANG II (10⁻¹¹ to 10⁻⁷ M) consistently depolarized OMDVR and induced Ψm oscillations at lower concentrations. The Cl⁻ channel blockers anthracene-9-decarboxylate (1 mM) and niflumic acid (10 μM) hyperpolarized resting pericytes and repolarized ANG II-treated pericytes. In voltage-clamp experiments, ANG II-treated pericytes exhibited slowly activating currents that were nearly eliminated by treatment with niflumic acid (10 μM) or removal of extracellular Ca²⁺. Those currents reversed at −31 and −10 mV when extracellular Cl⁻ concentration was 152 and 34 mM, respectively. In pericytes held at −70 mV, oscillating inward currents were sometimes observed; the reversal potential also shifted with extracellular Cl⁻ concentration. We conclude that ANG II activates a Ca²⁺-dependent Cl⁻ conductance in OMDVR pericytes to induce membrane depolarization and Ψm oscillations.