Failure of germinal center formation and impairment of response to endotoxin in tumor necrosis factor alpha-deficient mice.
T Taniguchi, M Takata, A Ikeda, E Momotani… - … ; a journal of technical …, 1997 - europepmc.org
T Taniguchi, M Takata, A Ikeda, E Momotani, K Sekikawa
Laboratory investigation; a journal of technical methods and pathology, 1997•europepmc.orgTo study the pathophysiologic roles of TNF alpha, we produced TNF alpha gene-disrupted
mice by gene targeting. TNF alpha-deficient mice develop normally without any alteration in
the lymphocyte populations. However, in these mice, the germinal center formation in the
peripheral lymphoid organs failed in response to the T cell-dependent antigens. TNF alpha-
deficient mice are resistant to lethal doses of endotoxin and D-galactosamine without
hepatocyte apoptosis, yet demonstrate thymus apoptosis. Our results indicated an important …
mice by gene targeting. TNF alpha-deficient mice develop normally without any alteration in
the lymphocyte populations. However, in these mice, the germinal center formation in the
peripheral lymphoid organs failed in response to the T cell-dependent antigens. TNF alpha-
deficient mice are resistant to lethal doses of endotoxin and D-galactosamine without
hepatocyte apoptosis, yet demonstrate thymus apoptosis. Our results indicated an important …
To study the pathophysiologic roles of TNF alpha, we produced TNF alpha gene-disrupted mice by gene targeting. TNF alpha-deficient mice develop normally without any alteration in the lymphocyte populations. However, in these mice, the germinal center formation in the peripheral lymphoid organs failed in response to the T cell-dependent antigens. TNF alpha-deficient mice are resistant to lethal doses of endotoxin and D-galactosamine without hepatocyte apoptosis, yet demonstrate thymus apoptosis. Our results indicated an important role for TNF alpha in germinal center formation and in the sepsis-induced hepatocyte apoptosis that precedes liver failure.
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