Activation of the bone-derived latent TGF beta complex by isolated osteoclasts

ROC Oreffo, GR Mundy, SM Seyedin… - … and biophysical research …, 1989 - Elsevier
ROC Oreffo, GR Mundy, SM Seyedin, LF Bonewald
Biochemical and biophysical research communications, 1989Elsevier
Although TGF beta is unquestionably an important growth regulatory polypeptide with effects
on many cell types, the cellular mechanisms which release it from the binding proteins which
mask its biological activity are not well understood. Here we show that when isolated
osteoclasts are activated, they release active TGF beta from the latent TGF beta complex
produced by bone organ cultures. Since active TGF beta has powerful inhibitory effects on
osteoclast formation and bone resorption and stimulates osteoblast activity, is present in …
Abstract
Although TGF beta is unquestionably an important growth regulatory polypeptide with effects on many cell types, the cellular mechanisms which release it from the binding proteins which mask its biological activity are not well understood. Here we show that when isolated osteoclasts are activated, they release active TGF beta from the latent TGF beta complex produced by bone organ cultures. Since active TGF beta has powerful inhibitory effects on osteoclast formation and bone resorption and stimulates osteoblast activity, is present in abundant amounts in the bone matrix and is released during hormone-stimulated osteoclastic bone resorption, the activation of TGF beta by stimulated osteoclasts may be an important regulatory step in normal bone remodeling.
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