Cisplatin generates superoxide anion by interaction with DNA in a cell-free system
H Masuda, T Tanaka, U Takahama - Biochemical and biophysical research …, 1994 - Elsevier
H Masuda, T Tanaka, U Takahama
Biochemical and biophysical research communications, 1994•ElsevierAbstract Cisplatin [cis-Diamminedichloroplatinum (II), DDP] generated superoxide anion (O−
2), as measured by chemiluminescence from a Cypridina luciferin analog (CLA), by
interaction with calf thymus DNA (ct-DNA) in phosphate-buffered saline (PBS) at PH 7.6, The
intensity of chemiluminescence of CLA (CCLA) increased with increased concentration of
DDP, ct-DNA or CLA in the limited range of concentrations of these agents tested. DDP-
induced CCLA was completely inhibited by the addition of an O− 2 scavenger, superoxide …
2), as measured by chemiluminescence from a Cypridina luciferin analog (CLA), by
interaction with calf thymus DNA (ct-DNA) in phosphate-buffered saline (PBS) at PH 7.6, The
intensity of chemiluminescence of CLA (CCLA) increased with increased concentration of
DDP, ct-DNA or CLA in the limited range of concentrations of these agents tested. DDP-
induced CCLA was completely inhibited by the addition of an O− 2 scavenger, superoxide …
Abstract
Cisplatin [cis-Diamminedichloroplatinum (II), DDP] generated superoxide anion (O−2), as measured by chemiluminescence from a Cypridina luciferin analog (CLA), by interaction with calf thymus DNA (ct-DNA) in phosphate-buffered saline (PBS) at PH 7.6, The intensity of chemiluminescence of CLA (CCLA) increased with increased concentration of DDP, ct-DNA or CLA in the limited range of concentrations of these agents tested. DDP-induced CCLA was completely inhibited by the addition of an O−2 scavenger, superoxide dismutase (SOD), or an antioxidant, ascorbic acid. Heat-inactivated SOD did not affect CCLA. The hydroxyl radical (OH·) scavengers, mannitol, ethanol, and formic acid, also caused a partial suppression of CCLA, suggesting that DDP produced OH· as well as O−2 in the cell-free system used.
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