[HTML][HTML] Quantitative mass spectrometry of histones H3. 2 and H3. 3 in Suz12-deficient mouse embryonic stem cells reveals distinct, dynamic post-translational …
SUZ12 is a core component of the polycomb repressive complex 2 (PRC2) and is required
for the differentiation of mouse embryonic stem cells (ESCs). PRC2 is associated with
transcriptional repression via methylation of H3 Lys-27. We applied quantitative mass
spectrometry to investigate the effects of Suz12 deficiency on H3. 2 and H3. 3 from mouse
ESCs. Using high mass accuracy MS combined with CID or electron transfer dissociation
(ETD) tandem mass spectrometry, we identified a total of 81 unique modified peptides from …
for the differentiation of mouse embryonic stem cells (ESCs). PRC2 is associated with
transcriptional repression via methylation of H3 Lys-27. We applied quantitative mass
spectrometry to investigate the effects of Suz12 deficiency on H3. 2 and H3. 3 from mouse
ESCs. Using high mass accuracy MS combined with CID or electron transfer dissociation
(ETD) tandem mass spectrometry, we identified a total of 81 unique modified peptides from …