A simplified method for the clinical-scale generation of central memory-like CD8+ T cells after transduction with lentiviral vectors encoding antitumor antigen T-cell …
S Yang, ME Dudley, SA Rosenberg… - Journal of …, 2010 - journals.lww.com
Adoptive transfer of antigen-specific CD8+ T cells can effectively treat patients with
metastatic melanoma. Recent efforts have emphasized the in vitro generation of antitumor T
cells by transduction of genes encoding antitumor T-cell receptors. At present, lentiviral
vector-mediated transduction of CD8+ T cells relies on anti-CD3/CD28 bead stimulation;
however, this method fails to efficiently expand CD8+ T cells. Herein we sought to establish
a methodology for lentiviral vector transduction using optimal activating agents for efficient …
metastatic melanoma. Recent efforts have emphasized the in vitro generation of antitumor T
cells by transduction of genes encoding antitumor T-cell receptors. At present, lentiviral
vector-mediated transduction of CD8+ T cells relies on anti-CD3/CD28 bead stimulation;
however, this method fails to efficiently expand CD8+ T cells. Herein we sought to establish
a methodology for lentiviral vector transduction using optimal activating agents for efficient …