Alterations to the structure of the glomerular filtration barrier lead to effacement of podocyte
foot processes, leakage of albumin, and the development of proteinuria. To better
understand the signaling pathways involved in the response of the glomerular filtration
barrier to injury, we studied freshly isolated rat glomeruli, which allows for the monitoring
and pharmacologic manipulation of early signaling events. Administration of protamine
sulfate rapidly damaged the isolated glomeruli, resulting in foot process effacement and …