[HTML][HTML] Complete reconstitution of human IκB kinase (IKK) complex in yeast: assessment of its stoichiometry and the role of IKKγ on the complex activity in the …
BS Miller, E Zandi - Journal of Biological Chemistry, 2001 - ASBMB
The IκB kinase (IKK) complex, composed of two catalytic subunits (IKKα and IKKβ) and a
regulatory subunit (IKKγ), is the key enzyme in activation of nuclear factor κB (NF-κB). To
study the mechanism and structure of the complex, we wanted to recombinantly express IKK
in a model organism that lacks IKK. For this purpose, we have recombinantly reconstituted
all three subunits together in yeast and have found that it is biochemically similar to IKK
isolated from human cells. We show that there is one regulatory subunit per kinase subunit …
regulatory subunit (IKKγ), is the key enzyme in activation of nuclear factor κB (NF-κB). To
study the mechanism and structure of the complex, we wanted to recombinantly express IKK
in a model organism that lacks IKK. For this purpose, we have recombinantly reconstituted
all three subunits together in yeast and have found that it is biochemically similar to IKK
isolated from human cells. We show that there is one regulatory subunit per kinase subunit …