[HTML][HTML] Oxidation of IκBα at methionine 45 is one cause of taurine chloramine-induced inhibition of NF-κB activation
A Kanayama, J Inoue, Y Sugita-Konishi… - Journal of Biological …, 2002 - ASBMB
A band shift of IκBα was observed in Western blots with Jurkat cells treated with 1 mm
taurine chloramine (TauCl) for 1 h. TauCl treatment inhibited tumor necrosis factor α (TNFα)-
initiated nuclear factor κB (NF-κB) activation. TauCl did not inhibit either the upstream of IκB
kinase (IKK) activation or IKK itself but did inhibit NF-κB activation induced by IKK
overexpression. Deletion experiments showed that a TauCl modification site causing the
band shift of IκBα is Met 45. High performance liquid chromatography and mass …
taurine chloramine (TauCl) for 1 h. TauCl treatment inhibited tumor necrosis factor α (TNFα)-
initiated nuclear factor κB (NF-κB) activation. TauCl did not inhibit either the upstream of IκB
kinase (IKK) activation or IKK itself but did inhibit NF-κB activation induced by IKK
overexpression. Deletion experiments showed that a TauCl modification site causing the
band shift of IκBα is Met 45. High performance liquid chromatography and mass …