Regulated secretion of Zn²⁺ from isolated pancreatic β-cells was imaged using laser-scanning confocal microscopy. In the method, β-cells were incubated in a solution containing the novel fluorescent Zn²⁺ indicator FluoZin-3. Zn²⁺ released from the cells reacted with the dye to form a fluorescent product, which was detected by the confocal microscope. The new dye is much brighter than Zinquin, previously used for this application, allowing detection limits of 10−40 nM and temporal resolution of 16 ms/image. The high temporal resolution allowed imaging of isolated fluorescent transients that occurred at the edge of the cells following stimulation with 20 mM glucose or 40 mM K⁺. Fluorescent transients took 16−50 ms to reach a peak from the initial rise and returned to baseline after 170 ± 50 ms (n = 78 transients from 15 cells). It was concluded that the transients correspond to detection of exocytotic release of Zn²⁺. Analysis of the temporal and spatial dispersion of the transients indicates that the release of Zn²⁺ is not diffusion limited but is instead kinetically controlled in agreement with previous observations of insulin release detected by amperometry.