[HTML][HTML] Stable Inducible Expression of a Functional Rat Liver Organic Anion Transport Protein in HeLa Cells (∗)

X Shi, S Bai, AC Ford, RD Burk, E Jacquemin… - Journal of Biological …, 1995 - ASBMB
Recently we expression cloned a rat liver organic anion transport protein in Xenopus laevis
oocytes (Jacquemin, E., Hagenbuch, B., Stieger, B., Wolkoff, AW, and Meier, PJ (1994) Proc.
Natl. Acad. Sci. USA 91, 133-137). In the present study, we have stably transfected the cDNA
encoding this protein into HeLa cells by using a vector containing a zinc-inducible promoter.
The parent cells have virtually no baseline transport of [35 S] sulfobromophthalein, whereas
the induced transfected cells express a novel 74-kDa protein and avidly transport this ligand …