[HTML][HTML] Botulinum neurotoxin type C cleaves a single Lys-Ala bond within the carboxyl-terminal region of syntaxins

G Schiavo, CC Shone, MK Bennett, RH Scheller… - Journal of biological …, 1995 - ASBMB
Botulinum neurotoxin serotype C (BoNT/C) is a 150-kDa protein produced by Clostridium
botulinum, which causes animal botulism. In contrast to the other botulinum neurotoxins that
contain one atom of zinc, highly purified preparations of BoNT/C bind two atoms of zinc per
toxin molecule. BoNT/C is a zinc-endopeptidase that cleaves syntaxin 1A at the Lys 235-Ala
254 and syntaxin 1B at the Lys 252-Ala 253 peptide bonds, only when they are inserted into
a lipid bilayer. The other Lys-Ala bond present within the carboxyl-terminal region is not …