Role of an N-ethylmaleimide-sensitive transport component in promoting fusion of transport vesicles with cisternae of the Golgi stack
V Malhotra, L Orci, BS Glick, MR Block, JE Rothman - Cell, 1988 - cell.com
V Malhotra, L Orci, BS Glick, MR Block, JE Rothman
Cell, 1988•cell.comAn N-ethylmaleimide-sensitive transport component (NSF) has been purified on the basis of
its ability to support transport between Golgi cisternae. We now report that NSF is needed for
membrane fusion. Thus, when NSF is withheld from incubations of Golgi stacks with cytosol
and ATP, uncoated transport vesicles accumulate. Biochemical experiments confirm this
conclusion and reveal that NSF is needed to form the first of two previously described
prefusion complexes. NSF, therefore, acts within a cascade in which a vesicle-cisterna …
its ability to support transport between Golgi cisternae. We now report that NSF is needed for
membrane fusion. Thus, when NSF is withheld from incubations of Golgi stacks with cytosol
and ATP, uncoated transport vesicles accumulate. Biochemical experiments confirm this
conclusion and reveal that NSF is needed to form the first of two previously described
prefusion complexes. NSF, therefore, acts within a cascade in which a vesicle-cisterna …
Summary
An N-ethylmaleimide-sensitive transport component (NSF) has been purified on the basis of its ability to support transport between Golgi cisternae. We now report that NSF is needed for membrane fusion. Thus, when NSF is withheld from incubations of Golgi stacks with cytosol and ATP, uncoated transport vesicles accumulate. Biochemical experiments confirm this conclusion and reveal that NSF is needed to form the first of two previously described prefusion complexes. NSF, therefore, acts within a cascade in which a vesicle-cisterna complex is matured until it is competent for fusion. We suggest that this reflects the stepwise assembly of a multisubunit “fusion machine” following vesicle attachment.
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