[HTML][HTML] Cytosolic and membrane-associated proteins involved in the recruitment of AP-1 adaptors onto the trans-Golgi network
MNJ Seaman, PJ Sowerby, MS Robinson - Journal of Biological Chemistry, 1996 - ASBMB
The AP-1 adaptor complex is recruited from the cytosol onto the trans-Golgi network
membrane, where it co-assembles with clathrin into a coat that drives vesicle budding. The
GTPase ARF1 has been shown to be required for AP-1 recruitment, and here we
demonstrate that we can reconstitute full GTPγS-dependent recruitment of adaptors onto an
enriched trans-Golgi network membrane fraction by adding purified AP-1 and recombinant
myristylated ARF1, indicating that these are the only soluble proteins required for binding …
membrane, where it co-assembles with clathrin into a coat that drives vesicle budding. The
GTPase ARF1 has been shown to be required for AP-1 recruitment, and here we
demonstrate that we can reconstitute full GTPγS-dependent recruitment of adaptors onto an
enriched trans-Golgi network membrane fraction by adding purified AP-1 and recombinant
myristylated ARF1, indicating that these are the only soluble proteins required for binding …