[HTML][HTML] Biochemical Mechanisms of the Generation of Endogenous Long Chain Ceramide in Response to Exogenous Short Chain Ceramide in the A549 Human …
B Ogretmen, BJ Pettus, MJ Rossi, R Wood… - Journal of Biological …, 2002 - ASBMB
Treatment of A549 cells with C 6-ceramide resulted in a significant increase in the
endogenous long chain ceramide levels, which was inhibited by fumonisin B1 (FB1), and
not by myriocin (MYR). The biochemical mechanisms of generation of endogenous
ceramide were investigated using A549 cells treated with selectively labeled C 6-
ceramides,[sphingosine-3-3 H] d-erythro-, and N-[N-hexanoyl-1-14 C] d-erythro-C 6-
ceramide. The results demonstrated that 3 H label was incorporated into newly synthesized …
endogenous long chain ceramide levels, which was inhibited by fumonisin B1 (FB1), and
not by myriocin (MYR). The biochemical mechanisms of generation of endogenous
ceramide were investigated using A549 cells treated with selectively labeled C 6-
ceramides,[sphingosine-3-3 H] d-erythro-, and N-[N-hexanoyl-1-14 C] d-erythro-C 6-
ceramide. The results demonstrated that 3 H label was incorporated into newly synthesized …