[HTML][HTML] Molecular mechanisms of ceramide-mediated telomerase inhibition in the A549 human lung adenocarcinoma cell line
B Ogretmen, JM Kraveka, D Schady, J Usta… - Journal of Biological …, 2001 - ASBMB
This study was aimed at identifying the molecular mechanisms by which ceramide inhibits
telomerase activity in the A549 human lung adenocarcinoma cell line. C 6-ceramide (20 µm)
caused a significant reduction of telomerase activity at 24 h as detected using the telomeric
repeat amplification protocol, and this inhibition correlated with decreased telomerase
reverse transcriptase (hTERT) protein. Semi-quantitative reverse transcriptase-polymerase
chain reaction (RT-PCR) and Northern blot analyses showed that C 6-ceramide significantly …
telomerase activity in the A549 human lung adenocarcinoma cell line. C 6-ceramide (20 µm)
caused a significant reduction of telomerase activity at 24 h as detected using the telomeric
repeat amplification protocol, and this inhibition correlated with decreased telomerase
reverse transcriptase (hTERT) protein. Semi-quantitative reverse transcriptase-polymerase
chain reaction (RT-PCR) and Northern blot analyses showed that C 6-ceramide significantly …