Occurrence of a Large Ca2+‐Independent Release of Glutamate During Anoxia in Isolated Nerve Terminals (Synaptosomes)

J Sanchez‐Prieto, P González - Journal of neurochemistry, 1988 - Wiley Online Library
J Sanchez‐Prieto, P González
Journal of neurochemistry, 1988Wiley Online Library
Isolated rat cerebral cortical synaptosomes made anoxic by addition of cyanide developed
an inhibition of the Ca2+‐dependent release of glutamate 2 min after the addition of the
metabolic inhibitor when the intrasynaptosomal ATP/ADP ratio decreased below 1.7. In
contrast, cyanide induced a continuous efflux of glutamate through a Ca2+‐independent
pathway that accounted for the release of 25% of total intrasynaptosomal glutamate in 5 min.
The results suggest that a Ca2+‐independent release of glutamate could be implicated in …
Abstract
Isolated rat cerebral cortical synaptosomes made anoxic by addition of cyanide developed an inhibition of the Ca2+‐dependent release of glutamate 2 min after the addition of the metabolic inhibitor when the intrasynaptosomal ATP/ADP ratio decreased below 1.7. In contrast, cyanide induced a continuous efflux of glutamate through a Ca2+‐independent pathway that accounted for the release of 25% of total intrasynaptosomal glutamate in 5 min. The results suggest that a Ca2+‐independent release of glutamate could be implicated in the neurotoxic action of this amino acid during anoxia.
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