The mechanism of inactivation of human factor V and human factor Va by activated protein C.
M Kalafatis, MD Rand, KG Mann - Journal of Biological Chemistry, 1994 - ASBMB
The cleavage of human factor V and human factor Va by human activated protein C (APC)
was analyzed in the absence and presence of phospholipid vesicles containing 75%
phosphatidylcholine (PC) and 25% phosphatidylserine (PS). Membrane-bound human
factor V (250 nM) is cleaved by APC (2.5 nM) to give M (r)= 200,000, 70,000, 45,000, and
30,000 fragments and an M (r)= 22/20,000 doublet. These fragments are released after four
sequential cleavages of the membrane-bound procofactor at Arg306, Arg506, Arg679, and …
was analyzed in the absence and presence of phospholipid vesicles containing 75%
phosphatidylcholine (PC) and 25% phosphatidylserine (PS). Membrane-bound human
factor V (250 nM) is cleaved by APC (2.5 nM) to give M (r)= 200,000, 70,000, 45,000, and
30,000 fragments and an M (r)= 22/20,000 doublet. These fragments are released after four
sequential cleavages of the membrane-bound procofactor at Arg306, Arg506, Arg679, and …