[HTML][HTML] Evaluation of the initiation phase of blood coagulation using ultrasensitive assays for serine proteases
S Butenas, C van't Veer, KG Mann - Journal of Biological Chemistry, 1997 - ASBMB
The initiation phase of enzyme generation in a reconstituted model of the tissue factor (TF)
pathway to thrombin was evaluated. At 1.25 pm added TF, no thrombin generation was
observed in the absence of factor V. The substitution of factor Va for factor V increased the
rate of thrombin generation. Factor X activation during the initiation phase was not
influenced by the absence of factor VIII or thrombin, leading to the conclusion that initially
factor Xa is generated exclusively by the factor VIIa-TF complex. When thrombin was …
pathway to thrombin was evaluated. At 1.25 pm added TF, no thrombin generation was
observed in the absence of factor V. The substitution of factor Va for factor V increased the
rate of thrombin generation. Factor X activation during the initiation phase was not
influenced by the absence of factor VIII or thrombin, leading to the conclusion that initially
factor Xa is generated exclusively by the factor VIIa-TF complex. When thrombin was …