Signalling specificity in eukaryotic cells is maintained by several mechanisms. One
mechanism by which mitogen-activated protein (MAP) kinases ensure their specificity of
action is by interacting with their substrates through docking domains. These docking
domains recruit the kinases to the correct substrates and enhance their fidelity and efficiency
of action. Additional specificity determinants in the substrates serve to enhance the
specificity of substrate phosphorylation by MAP kinases further.