Flow cytometric detection of viruses
CPD Brussaard, D Marie, G Bratbak - Journal of virological methods, 2000 - Elsevier
Representatives from several different virus families (Baculoviridae, Herpesviridae,
Myoviridae, Phycodnaviridae, Picornaviridae, Podoviridae, Retroviridae, and Siphoviridae)
were stained using a variety of highly fluorescent nucleic acid specific dyes (SYBR Green I,
SYBR Green II, OliGreen, PicoGreen) and examined using a standard flow cytometer
equipped with a standard 15 mW argon-ion laser. The highest green fluorescence
intensities were obtained using SYBR Green I. DNA viruses with genome sizes between …
Myoviridae, Phycodnaviridae, Picornaviridae, Podoviridae, Retroviridae, and Siphoviridae)
were stained using a variety of highly fluorescent nucleic acid specific dyes (SYBR Green I,
SYBR Green II, OliGreen, PicoGreen) and examined using a standard flow cytometer
equipped with a standard 15 mW argon-ion laser. The highest green fluorescence
intensities were obtained using SYBR Green I. DNA viruses with genome sizes between …