[HTML][HTML] Phosphorylation and activation of heart 6-phosphofructo-2-kinase by protein kinase B and other protein kinases of the insulin signaling cascades
To understand the insulin-induced activation of 6-phosphofructo-2-kinase (PFK-2) of the
bifunctional enzyme PFK-2/fructose-2, 6-bisphosphatase in heart, the effect of
phosphorylation by protein kinases of the insulin signaling pathways on PFK-2 activity was
studied. Purified PFK-2/fructose-2, 6-bisphosphatase from bovine heart is a mixture of two
isoforms (M r 58,000 and 54,000 on SDS-polyacrylamide gels). The M r 54,000 protein is an
alternatively spliced form, lacking phosphorylation sites for protein kinases. Recombinant …
bifunctional enzyme PFK-2/fructose-2, 6-bisphosphatase in heart, the effect of
phosphorylation by protein kinases of the insulin signaling pathways on PFK-2 activity was
studied. Purified PFK-2/fructose-2, 6-bisphosphatase from bovine heart is a mixture of two
isoforms (M r 58,000 and 54,000 on SDS-polyacrylamide gels). The M r 54,000 protein is an
alternatively spliced form, lacking phosphorylation sites for protein kinases. Recombinant …