Activation of the p53 tumor suppressor by cellular stress leads to variable responses ranging
from growth inhibition to apoptosis. TIGAR is a novel p53-inducible gene that inhibits
glycolysis by reducing cellular levels of fructose-2, 6-bisphosphate, an activator of glycolysis
and inhibitor of gluconeogenesis. Here we describe structural and biochemical studies of
TIGAR from Danio rerio. The overall structure forms a histidine phosphatase fold with a
phosphate molecule coordinated to the catalytic histidine residue and a second phosphate …