13C MR spectroscopy measurements of glutaminase activity in human hepatocellular carcinoma cells using hyperpolarized 13C‐labeled glutamine

FA Gallagher, MI Kettunen, SE Day… - … in Medicine: An …, 2008 - Wiley Online Library
Magnetic Resonance in Medicine: An Official Journal of the …, 2008Wiley Online Library
Dynamic nuclear polarization (DNP) is an emerging technique for increasing the sensitivity
of 13C MR spectroscopy (MRS).[5‐13C1] Glutamine was hyperpolarized using this
technique by up to 5%, representing a 6000‐fold increase in sensitivity. The conversion of
hyperpolarized glutamine to glutamate by mitochondrial glutaminase was demonstrated
using 13C‐MRS measurements in cultured human hepatoma cells (HepG2). These results
represent the first step in developing an imaging technique for detecting glutamine …
Abstract
Dynamic nuclear polarization (DNP) is an emerging technique for increasing the sensitivity of 13C MR spectroscopy (MRS). [5‐13C1]Glutamine was hyperpolarized using this technique by up to 5%, representing a 6000‐fold increase in sensitivity. The conversion of hyperpolarized glutamine to glutamate by mitochondrial glutaminase was demonstrated using 13C‐MRS measurements in cultured human hepatoma cells (HepG2). These results represent the first step in developing an imaging technique for detecting glutamine metabolism in vivo. Furthermore, since glutamine utilization has been correlated with cell proliferation, the study suggests a new technique for detecting changes in tumor cell proliferation. Magn Reson Med 60:253–257, 2008. © 2008 Wiley‐Liss, Inc.
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