[HTML][HTML] Comparative metabolic flux profiling of melanoma cell lines: beyond the Warburg effect

DA Scott, AD Richardson, FV Filipp, CA Knutzen… - Journal of Biological …, 2011 - ASBMB
Metabolic rewiring is an established hallmark of cancer, but the details of this rewiring at a
systems level are not well characterized. Here we acquire this insight in a melanoma cell
line panel by tracking metabolic flux using isotopically labeled nutrients. Metabolic profiling
and flux balance analysis were used to compare normal melanocytes to melanoma cell lines
in both normoxic and hypoxic conditions. All melanoma cells exhibited the Warburg
phenomenon; they used more glucose and produced more lactate than melanocytes. Other …