[HTML][HTML] Interaction of the xanthine nucleotide binding Goα mutant with G protein-coupled receptors
B Yu, MI Simon - Journal of Biological Chemistry, 1998 - ASBMB
We constructed a double mutant version of the α subunit of Go that was regulated by
xanthine nucleotides instead of guanine nucleotides (GoαX). We investigated the interaction
between GoαX and G protein-coupled receptors in vitro. First, we found that the activated m2
muscarinic cholinergic receptor (MAChR) could facilitate the exchange of XTPγS for XDP in
the GoαXβγ heterotrimer. Second, the GoαXβγ complex was able to induce the high affinity
ligand-binding state in the N-formyl peptide receptor (NFPR). These experiments …
xanthine nucleotides instead of guanine nucleotides (GoαX). We investigated the interaction
between GoαX and G protein-coupled receptors in vitro. First, we found that the activated m2
muscarinic cholinergic receptor (MAChR) could facilitate the exchange of XTPγS for XDP in
the GoαXβγ heterotrimer. Second, the GoαXβγ complex was able to induce the high affinity
ligand-binding state in the N-formyl peptide receptor (NFPR). These experiments …