[HTML][HTML] GDP Affinity and Order State of the Catalytic Site Are Critical for Function of Xanthine Nucleotide-selective GαsProteins
A Gille, K Wenzel-Seifert, MB Doughty… - Journal of Biological …, 2003 - ASBMB
Xanthine nucleotide-selective small GTP-binding proteins with an Asp/Asn mutation are
valuable for the analysis of individual GTP-binding proteins in complex systems. Similar
applications can be devised for heterotrimeric G-proteins. However, Asp/Asn mutants of Gα
o, Gα 11, and Gα 16 were inactive. An additional Gln/Leu mutation in the catalytic site,
reducing GTPase activity and increasing GDP affinity, was required to generate xanthine
nucleotide-selective unspecified G-protein α-subunit (Gα). Our study aim was to generate …
valuable for the analysis of individual GTP-binding proteins in complex systems. Similar
applications can be devised for heterotrimeric G-proteins. However, Asp/Asn mutants of Gα
o, Gα 11, and Gα 16 were inactive. An additional Gln/Leu mutation in the catalytic site,
reducing GTPase activity and increasing GDP affinity, was required to generate xanthine
nucleotide-selective unspecified G-protein α-subunit (Gα). Our study aim was to generate …