[HTML][HTML] Cyclic AMP signaling inhibits megakaryocytic differentiation by targeting transcription factor 3 (E2A) cyclin-dependent kinase inhibitor 1A (CDKN1A) …
JD Rubinstein, KE Elagib, AN Goldfarb - Journal of Biological Chemistry, 2012 - ASBMB
Signaling via the intracellular second messenger cyclic AMP (cAMP) has long been
implicated in the repression of megakaryocytic differentiation. However, the mechanisms by
which cAMP signaling impairs megakaryopoiesis have never been elucidated. In a human
CD34+ cell culture model, we show that the adenylyl cyclase agonist forskolin inhibits
megakaryocytic differentiation in a protein kinase A-dependent manner. Using this system to
screen for downstream effectors, we identified the transcription factor E2A as a key target in …
implicated in the repression of megakaryocytic differentiation. However, the mechanisms by
which cAMP signaling impairs megakaryopoiesis have never been elucidated. In a human
CD34+ cell culture model, we show that the adenylyl cyclase agonist forskolin inhibits
megakaryocytic differentiation in a protein kinase A-dependent manner. Using this system to
screen for downstream effectors, we identified the transcription factor E2A as a key target in …