The measurement of free fatty acid1 concentration with the fluorescent probe ADIFAB: A practical guide for the use of the ADIFAB probe

GV Richieri, RT Ogata, AM Kleinfeld - Lipid Binding Proteins within …, 1999 - Springer
GV Richieri, RT Ogata, AM Kleinfeld
Lipid Binding Proteins within Molecular and Cellular Biochemistry, 1999Springer
The aqueous phase monomers of fatty acids (FFA) appear in many steps of fat metabolism.
Understanding metabolism requires that accurate measurements of FFA levels be
determined in enzyme-mediated as well as in membrane and protein binding reactions.
Measuring long chain FFA levels with sufficient sensitivity and temporal resolution is now
possible using fluorescent probes constructed by ligating fluorescent groups and fatty acid
binding proteins. In this paper we provide a practical description of the use of ADIFAB, the …
Abstract
The aqueous phase monomers of fatty acids (FFA) appear in many steps of fat metabolism. Understanding metabolism requires that accurate measurements of FFA levels be determined in enzyme-mediated as well as in membrane and protein binding reactions. Measuring long chain FFA levels with sufficient sensitivity and temporal resolution is now possible using fluorescent probes constructed by ligating fluorescent groups and fatty acid binding proteins. In this paper we provide a practical description of the use of ADIFAB, the acrylodan labeled intestinal fatty acid binding protein. We describe with specific examples how ADIFAB can be used to determine, (1) FFA concentrations in aqueous solutions, (2) binding affinities of fatty acid binding proteins, (3) membrane/water partition coefficients, (4) lipase activities, and (5) serum levels of FFA. (Mol Cell Biochem 192: 87–94, 1999)
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