[HTML][HTML] The RIPE3b1 activator of the insulin gene is composed of a protein (s) of approximately 43 kDa, whose DNA binding activity is inhibited by protein …

L Zhao, MA Cissell, E Henderson, R Colbran… - Journal of Biological …, 2000 - ASBMB
Glucose-stimulated and pancreatic islet β cell-specific expression of the insulin gene is
mediated in part by the C1 DNA-element binding complex, termed RIPE3b1. In this report,
we define the molecular weight range of the protein (s) that compose this β cell-enriched
activator complex and show that protein phosphatase treatment inhibits RIPE3b1 DNA
binding activity. Fractionation of β cell nuclear extracts by sodium dodecyl sulfate-
polyacrylamide gel electrophoresis indicated that RIPE3b1 binding was mediated by a …