Induction of anti-melanoma CTL response using DC transfected with mutated mRNA encoding full-length Melan-A/MART-1 antigen with an A27L amino acid …
Z Abdel-Wahab, MF Kalady, S Emani, MW Onaitis… - Cellular …, 2003 - Elsevier
Modification of the parental immunodominant Melan-A/MART-1 peptide (MART-126–35) by
replacing the alanine with leucine (A27L) enhances its immunogenicity. Because of the
reported advantages of RNA over peptides in DC vaccines, we sought to mutate the MART-1
gene to encode a full-length MART-1 antigen with an A27L amino acid substitution. Human
DC were transfected with A27L-mutated MART-1 RNA (A27L RNA) or native MART-1 RNA,
and then used to stimulate autologous T cells from a series of 8 HLA-A2+ volunteers. After …
replacing the alanine with leucine (A27L) enhances its immunogenicity. Because of the
reported advantages of RNA over peptides in DC vaccines, we sought to mutate the MART-1
gene to encode a full-length MART-1 antigen with an A27L amino acid substitution. Human
DC were transfected with A27L-mutated MART-1 RNA (A27L RNA) or native MART-1 RNA,
and then used to stimulate autologous T cells from a series of 8 HLA-A2+ volunteers. After …