[HTML][HTML] In vitro and in vivo characterization of a novel semaphorin 3A inhibitor, SM-216289 or xanthofulvin

K Kikuchi, A Kishino, O Konishi, K Kumagai… - Journal of Biological …, 2003 - ASBMB
SM-216289 (xanthofulvin) isolated from the fermentation broth of a fungal strain, Penicillium
sp. SPF-3059, was identified as a strong semaphorin 3A (Sema3A) inhibitor. Sema3A-
induced growth cone collapse of dorsal root ganglion neurons in vitro was completely
abolished in the presence of SM-216289 at levels less than 2 μm (IC 50= 0.16 μm). When
dorsal root ganglion explants were co-cultured with Sema3A-producing COS7 cells in a
collagen gel matrix, SM-216289 enabled neurites to grow toward the COS7 cells. SM …