Selective gene silencing by RNA interference (RNAi) is a valuable tool for the targeted
manipulation of the development and/or function of cells. Using a fluorescein-labeled non-
silencing siRNA duplex, we established a protocol for the electroporation of primary mouse
macrophages which routinely yielded> 95% transfected cells. Electroporation of siRNAs
directed against MAPK1 and CD86 led to an efficient knock-down of cellular protein in bone
marrow-derived mouse macrophages (BM-Mϕ). Importantly, the electroporation procedure …