Uncoupling of expression of an intronic microRNA and its myosin host gene by exon skipping
ML Bell, M Buvoli, LA Leinwand - Molecular and cellular biology, 2010 - Taylor & Francis
ML Bell, M Buvoli, LA Leinwand
Molecular and cellular biology, 2010•Taylor & FrancisThe ancient MYH7b gene, expressed in striated muscle and brain, encodes a sarcomeric
myosin and the intronic microRNA miR-499. We find that skipping of an exon introduces a
premature termination codon in the transcript that downregulates MYH7b protein production
without affecting microRNA expression. Among other genes, endogenous miR-499 targets
the 3′ untranslated region of the transcription factor Sox6, which in turn acts as a repressor
of MYH7b transcriptional activity. Thus, concerted transcription and alternative splicing …
myosin and the intronic microRNA miR-499. We find that skipping of an exon introduces a
premature termination codon in the transcript that downregulates MYH7b protein production
without affecting microRNA expression. Among other genes, endogenous miR-499 targets
the 3′ untranslated region of the transcription factor Sox6, which in turn acts as a repressor
of MYH7b transcriptional activity. Thus, concerted transcription and alternative splicing …
The ancient MYH7b gene, expressed in striated muscle and brain, encodes a sarcomeric myosin and the intronic microRNA miR-499. We find that skipping of an exon introduces a premature termination codon in the transcript that downregulates MYH7b protein production without affecting microRNA expression. Among other genes, endogenous miR-499 targets the 3′ untranslated region of the transcription factor Sox6, which in turn acts as a repressor of MYH7b transcriptional activity. Thus, concerted transcription and alternative splicing uncouple the level of expression of MYH7b and miR-499 when their coexpression is not required.
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