Effects of chronic electrical stimulation on myosin heavy chain expression in satellite cell cultures derived from rat muscles of different fiber-type composition
U Wehrle, S Düsterhöft, D Pette - Differentiation, 1994 - Elsevier
Myotube cultures were established from satellite cells of three rat muscles of different fiber-
type composition, slow-twitch soleus, diaphragm, and fast-twitch tibialis anterior (TA). Effects
of chronic electrical stimulation were studied by exposing these cultures for up to 13 days to
a stimulus pattern consisting of 250 ms impulse trains of 40 Hz, repeated every 4 s. Changes
in myosin expression were assessed at the mRNA level by Northern blotting and in situ
hybridization. Expression of slow myosin at the protein level was analysed by …
type composition, slow-twitch soleus, diaphragm, and fast-twitch tibialis anterior (TA). Effects
of chronic electrical stimulation were studied by exposing these cultures for up to 13 days to
a stimulus pattern consisting of 250 ms impulse trains of 40 Hz, repeated every 4 s. Changes
in myosin expression were assessed at the mRNA level by Northern blotting and in situ
hybridization. Expression of slow myosin at the protein level was analysed by …