Dissociation of double stranded polynucleotide helical structures by eukaryotic initiation factors, as revealed by a novel assay
TG Lawson, KA Lee, MM Maimone, RD Abramson… - Biochemistry, 1989 - ACS Publications
TG Lawson, KA Lee, MM Maimone, RD Abramson, TE Dever, WC Merrick, RE Thach
Biochemistry, 1989•ACS PublicationsMaterials and Methods Preparation of Reovirus, Globin mRNAs, and cDNAs. Capped
reovirus mRNAs were prepared by in vitro transcription from protease-activated reovirus
cores in the presence of [5, 6-3H] uridine5'-triphosphate (ICN) and purified as previously
described (Ray et al., 1985; Lawson et al., 1986). Rabbit globin mRNA was purified by using
the procedures described earlier (Brendler et al., 1981). Chemically synthesized
pentadecamer DNAs were prepared as described (Lawson et al., 1986). Purified DNAs and …
reovirus mRNAs were prepared by in vitro transcription from protease-activated reovirus
cores in the presence of [5, 6-3H] uridine5'-triphosphate (ICN) and purified as previously
described (Ray et al., 1985; Lawson et al., 1986). Rabbit globin mRNA was purified by using
the procedures described earlier (Brendler et al., 1981). Chemically synthesized
pentadecamer DNAs were prepared as described (Lawson et al., 1986). Purified DNAs and …
Materials and Methods
Preparation of Reovirus, Globin mRNAs, and cDNAs. Capped reovirus mRNAs were prepared by in vitro transcription from protease-activated reovirus cores in the presence of [5, 6-3H] uridine5'-triphosphate (ICN) and purified as previously described (Ray et al., 1985; Lawson et al., 1986). Rabbit globin mRNA was purified by using the procedures described earlier (Brendler et al., 1981). Chemically synthesized pentadecamer DNAs were prepared as described (Lawson et al., 1986). Purified DNAs and oligoriboadenylic acid (12-18 residues in length, Pharmacia) were labeled at the 5'end with [-32] and T4 polynucleotide kinase
ACS Publications