Unusual phenotype of B cells in the thymus of normal mice.
M Miyama-Inaba, S Kuma, K Inaba, H Ogata… - The Journal of …, 1988 - rupress.org
M Miyama-Inaba, S Kuma, K Inaba, H Ogata, H Iwai, R Yasumizu, S Muramatsu…
The Journal of experimental medicine, 1988•rupress.orgA small number of B cells are found in the thymus of normal mice. A population of B
lymphocytes could be enriched to greater than 90% purity by isolating a low-density fraction
on Percoll density gradients and then depleting T cells with a mixture of anti-Thy-1, CD4,
and CD8 mAbs and complement. Enrichment was monitored by surface Ig staining and by
functional studies (responsiveness to LPS, and to anti-mu plus IL-4). When the phenotype of
these B cells was studied by flow cytometry, 60-80% had the phenotype Ly-1+(CD5), Ia+ …
lymphocytes could be enriched to greater than 90% purity by isolating a low-density fraction
on Percoll density gradients and then depleting T cells with a mixture of anti-Thy-1, CD4,
and CD8 mAbs and complement. Enrichment was monitored by surface Ig staining and by
functional studies (responsiveness to LPS, and to anti-mu plus IL-4). When the phenotype of
these B cells was studied by flow cytometry, 60-80% had the phenotype Ly-1+(CD5), Ia+ …
A small number of B cells are found in the thymus of normal mice. A population of B lymphocytes could be enriched to greater than 90% purity by isolating a low-density fraction on Percoll density gradients and then depleting T cells with a mixture of anti-Thy-1, CD4, and CD8 mAbs and complement. Enrichment was monitored by surface Ig staining and by functional studies (responsiveness to LPS, and to anti-mu plus IL-4). When the phenotype of these B cells was studied by flow cytometry, 60-80% had the phenotype Ly-1+ (CD5), Ia+, B220low (CD45R), and Mac-1+ (CD 11b). In contrast, splenic B cells lacked CD5 and CD11b and expressed higher levels of B220 and Ia antigens. These results indicate that most thymic B cells have the phenotype of the Ly-1 B cell subset, which was identified previously as a trace subpopulation in some peripheral tissues and is thought to play a role in autoantibody formation.
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