A modified choline-deficient, ethionine-supplemented diet protocol effectively induces oval cells in mouse liver

B Akhurst, EJ Croager, CA Farley-Roche, JK Ong… - Hepatology, 2001 - Elsevier
Several reliable and reproducible methods are available to induce oval cells in rat liver.
Effective methods often involve inhibiting proliferation in hepatocytes using an alkylating
agent, then subjecting the rat to partial hepatectomy (PH). The surgery is difficult to perform
reproducibly in mice. Approaches that do not include partial hepatectomy, such as
administration of D-galactosamine, are ineffective in mice. We found that a choline-deficient,
ethionine-supplemented (CDE) diet, which is very effective in rats, leads to high morbidity …