Isolation of Oval Cells From Long–Evans Cinnamon Rats and Their Transformation Into HepatocytesIn VivoIn the Rat Liver
O Yasui, N Miura, K Terada, Y Kawarada, K Koyama… - Hepatology, 1997 - journals.lww.com
Oval cells function as compensatory cells in severe liver injury and are thought to be
equivalent to liver stem/progenitor cells. We isolated oval cells from the liver of Long–Evans
Cinnamon (LEC) rats by isopyknic centrifugation in a Percoll gradient. The cells were
gamma–glutamyl transpeptidase (GGTP)–positive, alpha–fetoprotein–positive, and
cytokeratin (CK) 18–and CK 19–positive, but albumin–negative in the cells. When oval cells
were transplanted to the liver, they were transformed into hepatocytes. To evaluate albumin …
equivalent to liver stem/progenitor cells. We isolated oval cells from the liver of Long–Evans
Cinnamon (LEC) rats by isopyknic centrifugation in a Percoll gradient. The cells were
gamma–glutamyl transpeptidase (GGTP)–positive, alpha–fetoprotein–positive, and
cytokeratin (CK) 18–and CK 19–positive, but albumin–negative in the cells. When oval cells
were transplanted to the liver, they were transformed into hepatocytes. To evaluate albumin …