Maintenance of differentiated phenotype of cultured rat hepatic lipocytes by basement membrane matrix
SL Friedman, FJ Roll, J Boyles, DM Arenson… - Journal of Biological …, 1989 - ASBMB
This study examined the role of extracellular matrix in regulating matrix phenotype of hepatic
lipocytes, the major source of matrix in liver. Lipocytes (Ito, stellate, or fat-storing cells) were
purified from normal rat liver and established in primary culture on either uncoated plastic,
plastic coated with individual matrix proteins, or a" complete" gel matrix, a basement
membrane-like matrix derived from the Engelbreth-Holm-Swarm (EHS) murine tumor. The
ultrastructure of lipocytes cultured on the gel matrix resembled that of cells in normal liver …
lipocytes, the major source of matrix in liver. Lipocytes (Ito, stellate, or fat-storing cells) were
purified from normal rat liver and established in primary culture on either uncoated plastic,
plastic coated with individual matrix proteins, or a" complete" gel matrix, a basement
membrane-like matrix derived from the Engelbreth-Holm-Swarm (EHS) murine tumor. The
ultrastructure of lipocytes cultured on the gel matrix resembled that of cells in normal liver …