Antineutrophil cytoplasmic autoantibodies (ANCA) recognizing a recombinant myeloperoxidase subunit
E Pedrollo, L Bleil, FA Bautz, JR Kalden… - … and Clinical Aspects, 1993 - Springer
E Pedrollo, L Bleil, FA Bautz, JR Kalden, EKF Bautz
ANCA-Associated Vasculitides: Immunological and Clinical Aspects, 1993•SpringerSera from 76 patients with diverse vasculitis, systemic lupus erythematosus (SLE) and
hydralazine-induced lupus (HiL) were analysed by ELISA for their reactivity with native,
reduced or urea-denatured MPO, respectively, as well as with bacterially expressed heavy
and light subunits of MPO. All sera (n= 20) recognizing native MPO showed a positive
reaction with reduced MPO, while 12 recognized the denatured protein. Most of the linear
epitopes are located in the light subunit, since 9 MPO-positive sera recognized significantly …
hydralazine-induced lupus (HiL) were analysed by ELISA for their reactivity with native,
reduced or urea-denatured MPO, respectively, as well as with bacterially expressed heavy
and light subunits of MPO. All sera (n= 20) recognizing native MPO showed a positive
reaction with reduced MPO, while 12 recognized the denatured protein. Most of the linear
epitopes are located in the light subunit, since 9 MPO-positive sera recognized significantly …
Summary
Sera from 76 patients with diverse vasculitis, systemic lupus erythematosus (SLE) and hydralazine-induced lupus (HiL) were analysed by ELISA for their reactivity with native, reduced or urea-denatured MPO, respectively, as well as with bacterially expressed heavy and light subunits of MPO. All sera (n=20) recognizing native MPO showed a positive reaction with reduced MPO, while 12 recognized the denatured protein. Most of the linear epitopes are located in the light subunit, since 9 MPO-positive sera recognized significantly the bacterially expressed, denatured light subunit, while only one serum recognized the bacterially expressed heavy subunit purified under denaturing conditions.
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