[HTML][HTML] CD69 suppresses sphingosine 1-phosophate receptor-1 (S1P1) function through interaction with membrane helix 4
AJ Bankovich, LR Shiow, JG Cyster - Journal of Biological Chemistry, 2010 - ASBMB
Lymphocyte egress from lymph nodes requires the G-protein-coupled sphingosine 1-
phosphate receptor-1 (S1P 1). The activation antigen CD69 associates with and inhibits the
function of S1P 1, inhibiting egress. Here we undertook biochemical characterization of the
requirements for S1P 1-CD69 complex formation. Domain swapping experiments between
CD69 and the related type II transmembrane protein, NKRp1A, identified a requirement for
the transmembrane and membrane proximal domains for specific interaction. Mutagenesis …
phosphate receptor-1 (S1P 1). The activation antigen CD69 associates with and inhibits the
function of S1P 1, inhibiting egress. Here we undertook biochemical characterization of the
requirements for S1P 1-CD69 complex formation. Domain swapping experiments between
CD69 and the related type II transmembrane protein, NKRp1A, identified a requirement for
the transmembrane and membrane proximal domains for specific interaction. Mutagenesis …