RNA interference in keratinocytes and an organotypic model of human epidermis

CL Simpson, S Kojima, S Getsios - Epidermal cells: Methods and protocols, 2010 - Springer
CL Simpson, S Kojima, S Getsios
Epidermal cells: Methods and protocols, 2010Springer
Gene silencing approaches afford investigators the ability to gain important insight into the
normal functional requirements of specific epidermal proteins and promise to yield a
powerful therapeutic means to dampen the level of proteins that are mutated or frequently
overexpressed in skin disease. The efficient and tractable delivery of siRNAs into epidermal
keratinocytes is seminal to this process. Here, we describe techniques for transient and long-
term silencing of a representative gene product, namely desmoglein 1, in primary human …
Abstract
Gene silencing approaches afford investigators the ability to gain important insight into the normal functional requirements of specific epidermal proteins and promise to yield a powerful therapeutic means to dampen the level of proteins that are mutated or frequently overexpressed in skin disease. The efficient and tractable delivery of siRNAs into epidermal keratinocytes is seminal to this process. Here, we describe techniques for transient and long-term silencing of a representative gene product, namely desmoglein 1, in primary human epidermal keratinocytes maintained as submerged cultures or three-dimensional organotypic raft cultures. As a complement to epidermal-specific gene targeting strategies in mice, these technical approaches permit relatively rapid loss-of-function studies purely in keratinocytes without some of the potential influences present in situ, such as an immune system or vasculature.
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