[HTML][HTML] Purification of the serine palmitoyltransferase complex responsible for sphingoid base synthesis by using affinity peptide chromatography techniques
K Hanada, T Hara, M Nishijima - Journal of Biological Chemistry, 2000 - ASBMB
Serine palmitoyltransferase (SPT), a membrane-bound enzyme of the endoplasmic
reticulum, catalyzes the condensation of palmitoyl coenzyme A (CoA) andl-serine to produce
3-ketodihydrosphingosine. This enzyme contains at least two different subunits, named the
LCB1 and LCB2 proteins. In the present study, we expressed a FLAG-and His 6 peptide-
tagged version of the hamster LCB1 protein in a Chinese hamster ovary cell mutant strain
lacking the endogenous LCB1 subunit and purified SPT from the cells near to homogeneity …
reticulum, catalyzes the condensation of palmitoyl coenzyme A (CoA) andl-serine to produce
3-ketodihydrosphingosine. This enzyme contains at least two different subunits, named the
LCB1 and LCB2 proteins. In the present study, we expressed a FLAG-and His 6 peptide-
tagged version of the hamster LCB1 protein in a Chinese hamster ovary cell mutant strain
lacking the endogenous LCB1 subunit and purified SPT from the cells near to homogeneity …
