Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1)
B7-H1 (PDL1) is a B7-related protein that inhibits T-cell responses. Human endothelial cells
(EC), which can support polyclonal stimulation (by anti-CD3 or Phytohemagglutinin (PHA))
or direct alloantigen stimulation of T cells, basally express B7-H1 and increase expression in
response to IFN-γ or coculture with allogeneic T cells. Previous studies have suggested that
endogenous B7-H1 on EC reduces T-cell responses. We engineered overexpression of B7-
H1 in EC (B7H1-EC) to evaluate whether this manipulation could reduce T-cell responses …
(EC), which can support polyclonal stimulation (by anti-CD3 or Phytohemagglutinin (PHA))
or direct alloantigen stimulation of T cells, basally express B7-H1 and increase expression in
response to IFN-γ or coculture with allogeneic T cells. Previous studies have suggested that
endogenous B7-H1 on EC reduces T-cell responses. We engineered overexpression of B7-
H1 in EC (B7H1-EC) to evaluate whether this manipulation could reduce T-cell responses …