[HTML][HTML] DAPK-1 binding to a linear peptide motif in MAP1B stimulates autophagy and membrane blebbing
B Harrison, M Kraus, L Burch, C Stevens… - Journal of biological …, 2008 - ASBMB
DAPK-1 (death-activated protein kinase) has wide ranging functions in cell growth control;
however, DAPK-1 interacting proteins that mediate these effects are not well defined. Protein-
protein interactions are driven in part by linear interaction motifs, and combinatorial peptide
libraries were used to identify peptide interfaces for the kinase domain of DAPK-1. Peptides
bound to DAPK-1core kinase domain fragments had homology to the N-terminal domain of
the microtubule-associated protein MAP1B. Immunobinding assays demonstrated that …
however, DAPK-1 interacting proteins that mediate these effects are not well defined. Protein-
protein interactions are driven in part by linear interaction motifs, and combinatorial peptide
libraries were used to identify peptide interfaces for the kinase domain of DAPK-1. Peptides
bound to DAPK-1core kinase domain fragments had homology to the N-terminal domain of
the microtubule-associated protein MAP1B. Immunobinding assays demonstrated that …