[HTML][HTML] Requirement of the mTOR kinase for the regulation of Maf1 phosphorylation and control of RNA polymerase III-dependent transcription in cancer cells
B Shor, J Wu, Q Shakey, L Toral-Barza, C Shi… - Journal of Biological …, 2010 - ASBMB
The mammalian target of rapamycin (mTOR) regulates growth via promoting translation and
transcription. Here, employing an mTOR active-site inhibitor WYE-125132 (WYE-132), we
have performed quantitative phospho-proteomics and identified a Ser-75-containing
phosphopeptide from Maf1, a known repressor of RNA polymerase III (Pol III) transcription.
Treatment of cancer cells with WYE-132 or the rapamycin analog CCI-779 led to a rapid loss
of the phosphorylation at Ser-75, whereas this effect was not seen in cells treated with …
transcription. Here, employing an mTOR active-site inhibitor WYE-125132 (WYE-132), we
have performed quantitative phospho-proteomics and identified a Ser-75-containing
phosphopeptide from Maf1, a known repressor of RNA polymerase III (Pol III) transcription.
Treatment of cancer cells with WYE-132 or the rapamycin analog CCI-779 led to a rapid loss
of the phosphorylation at Ser-75, whereas this effect was not seen in cells treated with …