Improved detection of simian immunodeficiency virus RNA by in situ hybridization in fixed tissue sections: combined effects of temperatures for tissue fixation and …
BA Fallert, TA Reinhart - Journal of virological methods, 2002 - Elsevier
In situ hybridization detection of viral RNAs in formaldehyde-fixed tissue specimens is used
frequently to characterize the extent of viral replication within host tissues. The ability to
determine the level of expression of viral RNAs in situ is dependent upon many factors
including the extent of cross-linking during fixation, the pretreatment regimen utilized to
relieve the effects of cross-linking, and the hybridization and wash protocols. In efforts to
improve our ability to detect cells infected productively by simian immunodeficiency virus …
frequently to characterize the extent of viral replication within host tissues. The ability to
determine the level of expression of viral RNAs in situ is dependent upon many factors
including the extent of cross-linking during fixation, the pretreatment regimen utilized to
relieve the effects of cross-linking, and the hybridization and wash protocols. In efforts to
improve our ability to detect cells infected productively by simian immunodeficiency virus …
