[HTML][HTML] Relative contribution of Nedd4 and Nedd4-2 to ENaC regulation in epithelia determined by RNA interference
PM Snyder, JC Steines, DR Olson - Journal of Biological Chemistry, 2004 - ASBMB
Epithelial Na+ transport is regulated in large part by mechanisms that control expression of
the epithelial Na+ channel (ENaC) at the cell surface. Nedd4 and Nedd4-2 are candidates to
control ENaC surface expression, but it is not known which of these proteins contributes to
ENaC regulation in epithelia. To address this question, we used RNA interference to
selectively reduce expression of Nedd4 or Nedd4-2. We found that endogenous Nedd4-2,
but not Nedd4, negatively regulates ENaC in two epithelial cell lines (Fischer rat thyroid and …
the epithelial Na+ channel (ENaC) at the cell surface. Nedd4 and Nedd4-2 are candidates to
control ENaC surface expression, but it is not known which of these proteins contributes to
ENaC regulation in epithelia. To address this question, we used RNA interference to
selectively reduce expression of Nedd4 or Nedd4-2. We found that endogenous Nedd4-2,
but not Nedd4, negatively regulates ENaC in two epithelial cell lines (Fischer rat thyroid and …