There is substantial evidence that proteinS-nitrosylation provides a significant route through
which nitric oxide (NO)-derived bioactivity is conveyed. However, most examples of S-
nitrosylation have been characterized on the basis of analysis in vitro, and relatively little
progress has been made in assessing the participant forms of nitric-oxide synthase (NOS) or
the dynamics of protein S-nitrosylationin situ. Here we utilize antibodies specific for the
nitrosothiol (SNO) moiety to provide an immunohistochemical demonstration that protein S …